Development of 6E3 antibody-mediated SERS immunoassay for drug-resistant influenza virus
نویسندگان
چکیده
Influenza viruses are responsible for several pandemics and seasonal epidemics pose a major public health threat. Even after outbreak, the emergence of drug-resistant influenza can disease control problems. Here we report novel 6E3 monoclonal antibody capable recognizing binding to H275Y neuraminidase (NA) mutation, which has been associated with reduced susceptibility NA inhibitors. The had K D 72.74 ?M wild-type 32.76 pM NA, suggesting that it identify pandemic H1N1 (pH1N1) virus. Molecular modeling studies also suggest high-affinity this pH1N1 NA. This was subject dot-blot, enzyme-linked immunosorbent assay, bare-eye detection, lateral flow assay demonstrate its specificity pH1N1. Furthermore, immobilized on Au nanoplate nanoparticles, enabling surface-enhanced Raman scattering (SERS)-based detection mutant Using antibody-mediated SERS immunoassay, virus be detected at low concentration 10 2 plaque-forming units/mL. We in human nasopharyngeal aspirate samples, 6E3-mediated potential diagnostic application. anticipate newly developed SERS-based immunoassay will contribute diagnosis improve treatment strategies patients. • against developed. Its determined. enabled aspirate.
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ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 2021
ISSN: ['0956-5663', '1873-4235']
DOI: https://doi.org/10.1016/j.bios.2021.113324